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Histology deals with the cell associations or tissues which, variously combined, form the body of plants and animals.

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It is actually the complement of gross anatomy and an indispensable preliminary for the understanding of physiology and pathology. Because of the difficulties encountered in the study of most vertebrate living cells the second procedure is the one generally followed in mammalian histology. The tissues of the mammalian body are: Blood and lymph; III.

The supporting tissues connective tissue; reticulo-endothelium; cartilage; bone ; IV. Discussion View source History. Histology and Embryology - Histology From Embryology. About Translations Nonidez JF.

Histology and Embryology 1941 - Histology

A commonly performed histochemical technique is the Perls Prussian blue reaction, used to demonstrate iron deposits in diseases like hemochromatosis. Histology samples have often been examined by radioactive techniques. In historadiography , a slide sometimes stained histochemically is X-rayed. More commonly, autoradiography is used to visualize the locations to which a radioactive substance has been transported within the body, such as cells in S phase undergoing DNA replication which incorporate tritiated thymidine , or sites to which radiolabeled nucleic acid probes bind in in situ hybridization.

For autoradiography on a microscopic level, the slide is typically dipped into liquid nuclear tract emulsion, which dries to form the exposure film. Individual silver grains in the film are visualized with dark field microscopy. Recently, antibodies have been used to specifically visualize proteins, carbohydrates, and lipids.

Medical Histology

This process is called immunohistochemistry , or when the stain is a fluorescent molecule, immunofluorescence. This technique has greatly increased the ability to identify categories of cells under a microscope. Other advanced techniques, such as nonradioactive in situ hybridization, can be combined with immunochemistry to identify specific DNA or RNA molecules with fluorescent probes or tags that can be used for immunofluorescence and enzyme-linked fluorescence amplification especially alkaline phosphatase and tyramide signal amplification. Fluorescence microscopy and confocal microscopy are used to detect fluorescent signals with good intracellular detail.

Digital cameras are increasingly used to capture histological and histopathological image. A Text and Atlas. The Nissl method and Golgi's method are useful in identifying neurons. Plastic embedding is commonly used in the preparation of material for electron microscopy. Tissues are embedded in epoxy resin.

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Very thin sections less than 0. The sections are stained with electron dense stains uranium and lead so that they can be seen with the electron microscope. Artifacts are structures or features in tissue that interfere with normal histological examination.

Histology | Definition of Histology by Merriam-Webster

These are not always present in normal tissue and can come from outside sources. Artifacts interfere with histology by changing the tissues appearance and hiding structures. These can be divided into two categories:. These are features and structures that have been introduced prior to the collection of the tissues.


A common example of these include: Artifacts can result from tissue processing. Processing commonly leads to changes like shrinkage, washing out of particular cellular components, color changes in different tissues types and alterations of the structures in the tissue. Because these are caused in a laboratory the majority of post histology artifacts can be avoided or removed after being discovered. A common example is mercury pigment left behind after using Zenker's fixative to fix a section.

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  7. Normal patterning of tissues and artifacts resulting from the tissue preparation process ensure that each histological section is unique. Like a piece of biological art these images provide a deep insight into the organization and function of our bodies.

    Histological patterns that look like everyday objects or features are emerging on social and scientific communities [15] and even in histopathology journal articles. It demonstrates that histology can be appreciated by not only the detail-oriented pathologist but also the art loving layperson and is making histology and pathology more accessible and less daunting as a complex science. From Wikipedia, the free encyclopedia. Not to be confused with Historiography. This article needs additional citations for verification. Please help improve this article by adding citations to reliable sources.

    Unsourced material may be challenged and removed. Marcello Malpighi and the Evolution of Embryology. A Brief Survey of Sources". The Genesis of Cancer: A Study in the History of Ideas. Johns Hopkins University Press. Most of Bichat's twenty-one tissues can be subsumed under the four categories generally accepted by contemporary histologists; epithelium, connective tissue, muscle, and nerve. Four of Bichat's tissues fall under the heading of epithelium epidermoid, mucous, serous, and synovial ; six under connective tissue dermoid, fibrous, fibrocartilaginous, cartilaginous, osseous, and cellular ; two under muscle; and two under nerve — the distinction between nervous governing "animal" life and nervous governing "organic" life corresponds with that between the voluntary and involuntary nervous systems.

    The arteries and the veins, long sources of contention, are classified today as compound tissues. The absorbents and the exhalants which Bichat thought to be open-ended vessels have dropped out or been replaced by the lymphatics. His medullary system has no counterpart among the present-day tissues. The University of Chicago Press. International Journal of Surgical Pathology. Simple squamous epithelium Endothelium Mesothelium Simple cuboidal epithelium Simple columnar epithelium Pseudostratified columnar epithelium Respiratory epithelium Stratified squamous epithelium Stratified cuboidal epithelium Stratified columnar epithelium Transitional epithelium Urothelium.

    Tubular gland Alveolar gland. Serous glands Mucous glands. Myoepithelial cell Serous demilune Ducts: Adherens junction Cadherin Desmosome Desmoglein Ion channels: Desmosome Desmoplakin Plakoglobin Tonofibril other membrane proteins: Soft tissue Fibrosis Scarring. Reticular Adipose Brown White. Calmodulin Vascular smooth muscle.

    Sarcospan Laminin, alpha 2. Epimysium Fascicle Perimysium Endomysium Connective tissue in skeletal muscle. Neuromuscular junction Motor unit Muscle spindle Excitation—contraction coupling Sliding filament mechanism. Myocardium Intercalated disc Nebulette. Desmin Sarcoplasm Sarcolemma T-tubule Sarcoplasmic reticulum.

    Myotilin Telethonin Dysferlin Fukutin Fukutin-related protein. Pyramidal Purkinje Granule Spindle Interneuron.

    Epithelial Tissue Review & Practice

    Astrocyte Ependymal cells Tanycyte Microglia. Retrieved from " https: Histology Histotechnology Staining Histochemistry Anatomy. Views Read Edit View history. In other projects Wikimedia Commons. This page was last edited on 8 February , at By using this site, you agree to the Terms of Use and Privacy Policy. Keratin—orange Cartilage—blue Bone matrix—deep blue Muscle fibers—red.

    Heidenhain's AZAN trichrome stain. Elastic fibres—dark brown Mast cells granules—purple Smooth muscle—light blue. Periodic acid-Schiff stain PAS.